ZNF574 is a Quality Control Factor For Defective Ribosome Biogenesis Intermediates.

Eukaryotic ribosome assembly is an intricate process that involves four ribosomal RNAs, 80 ribosomal proteins, and over 200 biogenesis factors that take part in numerous interdependent steps. This complexity creates a large genetic space in which pathogenic mutations can occur. Dead-end ribosome intermediates that result from biogenesis errors are rapidly degraded, affirming the existence of quality control pathway(s) that monitor ribosome assembly. However, the factors that differentiate between on-path and dead-end intermediates are unknown. We engineered a system to perturb ribosome assembly in human cells and discovered that faulty ribosomes are degraded via the ubiquitin proteasome system. We identified ZNF574 as a key component of a novel quality control pathway, which we term the Ribosome Assembly Surveillance Pathway (RASP). Loss of ZNF574 results in the accumulation of faulty biogenesis intermediates that interfere with global ribosome production, further emphasizing the role of RASP in protein homeostasis and cellular health.

A FOXC2 inhibitor, MC-1-F2, as a therapeutic candidate for targeting EMT in castration-resistant prostate cancer.

Androgen deprivation therapy (ADT) is the major treatment option for advanced prostate cancer. However, prostate cancer can develop into androgen-independent castration-resistant prostate cancer (CRPC) which is resistant to ADT. An alternative treatment strategy for CRPC can be targeting the epithelial-mesenchymal transition (EMT). EMT is governed by a series of transcription factors of which forkhead box protein C2 (FOXC2) is a central mediator. Our previous research into the inhibition of FOXC2 in breast cancer cells lead to the discovery of MC-1-F2, the first direct inhibitor of FOXC2. In current study on CRPC, MC-1-F2 has shown a decrease in mesenchymal markers, inhibition of cancer stem cell (CSC) properties and decrease in invasive capabilities of CRPC cell lines. We have also demonstrated a synergistic effect between MC-1-F2 and docetaxel treatments, leading to a decrease in docetaxel dosage, suggesting the possible combination therapy of MC-1-F2 and docetaxel for the effective treatment of CRPC.

Perfectionism, Prolonged Stress Reactivity, and Depression: A Two-Wave Cross-Lagged Analysis.

Perfectionism is a vulnerability factor for a wide array of psychopathology. Despite much evidence suggesting dysregulated stress response as an intermediary process that links perfectionism to psychopathology, the lack of a cross-lagged examination deterred researchers from making causal interpretations. This study examined the directionality of effects among perfectionism dimensions, stress reactivity, and depression. A total of 189 participants at time 1 and 94 at time 2 completed an online survey that consisted of measures of perfectionism, stress reactivity, and depression, one month apart. Cross-lagged analysis results showed that personal standards perfectionism predicted later prolonged stress reactivity but not depression at time 2. Self-critical perfectionism predicted later depression but not prolonged stress reactivity at time 2. Rather, prolonged stress reactivity at time 1 predicted self-critical perfectionism at time 2. Findings suggest that perfectionism dimensions are distinct in creating a dysregulated stress process. Future studies could incorporate other stress-related variables (e.g., coping) to further explicate the stress-generation process, in conjunction with stress reactivity.

University Students Adjusting to COVID-19 Stressors: Exploratory and Confirmatory Factor Analyses of the COVID-19 Stressors Questionnaire.

The psychological effects of COVID-19 have been documented in the past year, but scarce literature exists on the nature of COVID-19 stressors. Using a random split sample of 1199 young adult university students, results of exploratory factor analyses (EFA) identified a four-factor structure in the COVID-19 Stressors Questionnaire (C19SQ), which were labeled Resource Constraints, Social Restrictions, Future Uncertainty, and Health Concerns. This model was supported by a confirmatory factor analysis (CFA) when run on the other split sample of 1139 university students. Higher levels of COVID-19 stress were positively associated with anxiety and depressive symptoms and negatively associated with sleep duration, sleep quality, and the number of exercise days. COVID-19 stress also uniquely predicted poor university adjustment. This study demonstrated the link between COVID-19 stressors and mental and physical health symptoms, thus providing support for conceptualizing the psychological impact of the pandemic as adjustment problems for some individuals.

Perfectionism and alcohol-related problems: The role of procrastination.

OBJECTIVE: College drinking is a significant individual and societal problem, and thus, identifying risk factors to alcohol-related problems has been an important line of inquiry. Adding to this rich literature, the current study examined whether perfectionism dimensions were associated with alcohol-related problems and whether a poor self-regulation process linked these associations. PARTICIPANTS: A total of 410 university students completed measures pertaining to perfectionism, procrastination, and negative consequences of alcohol use. METHODS: Parallel mediation models were tested. RESULTS: There was support for an indirect effect in the association between perfectionistic concerns and alcohol-related problems through susceptibility to temptation but not through pure procrastination or irrational procrastination. Perfectionistic strivings dimension was not associated with alcohol-related problems and this relation was not mediated by any procrastination dimensions. CONCLUSIONS: Building internal resources to better resist immediately gratifying yet long-run detrimental behavioral habits is important, especially so for highly self-critical students.

Profiles of mindfulness and difficulties in emotion regulation and links to work-family-school conflict.

Objective We explored latent profiles based on mindfulness and difficulties in emotion regulation scores, and investigated each profiles' relations to work-family-school conflict (WFSC). Participants: A total of 194 first year college students (64.4% women) participated in this study. Methods: Latent profile analysis was utilized. Results: Three profiles emerged, characterized as the "healthy" profile (57.5%), the "observant yet judgmental" profile (33.3%) and the "unhealthy without strategies" profile (9.2%). The "healthy" profile showed (a) significantly lower scores on all conflict domains compared to the "observant yet judgmental" profile, and (b) significantly lower scores on all behavior-based conflicts regardless of the domains, compared to the "unhealthy without strategies" profile. The difference between the "observant yet judgmental" profile and "unhealthy without strategies" profile appeared in family-school time. Results indicate that mindfulness and healthy emotion regulation capacity function as protective factors to WFSC. Conclusions: Our findings hold strength in explicating profiles that would otherwise have not been detected when exploring mindfulness and difficulties in emotion regulation independently.

Association of Self-Compassion With Suicidal Thoughts and Behaviors and Non-suicidal Self Injury: A Meta-Analysis.

Objectives: Self-compassion functions as a psychological buffer in the face of negative life experiences. Considering that suicidal thoughts and behaviors (STBs) and non-suicidal self-injury (NSSI) are often accompanied by intense negative feelings about the self (e.g., self-loathing, self-isolation), self-compassion may have the potential to alleviate these negative attitudes and feelings toward oneself. This meta-analysis investigated the associations of self-compassion with STBs and NSSI. Methods: A literature search finalized in August 2020 identified 18 eligible studies (13 STB effect sizes and seven NSSI effect sizes), including 8,058 participants. Two studies were longitudinal studies, and the remainder were cross-sectional studies. A random-effects meta-analysis was conducted using CMA 3.0. Subgroup analyses, meta-regression, and publication bias analyses were conducted to probe potential sources of heterogeneity. Results: With regard to STBs, a moderate effect size was found for self-compassion (r = -0.34, k = 13). Positively worded subscales exhibited statistically significant effect sizes: self-kindness (r = -0.21, k = 4), common humanity (r = -0.20, k = 4), and mindfulness (r = -0.15, k = 4). For NSSI, a small effect size was found for self-compassion (r = -0.29, k = 7). There was a large heterogeneity (I 2 = 80.92% for STBs, I 2 = 86.25% for NSSI), and publication bias was minimal. Subgroup analysis results showed that sample characteristic was a moderator, such that a larger effect size was witnessed in clinical patients than sexually/racially marginalized individuals, college students, and healthy-functioning community adolescents. Conclusions: Self-compassion was negatively associated with STBs and NSSI, and the effect size of self-compassion was larger for STBs than NSSI. More evidence is necessary to gauge a clinically significant protective role that self-compassion may play by soliciting results from future longitudinal studies or intervention studies.

Daily change patterns in mindfulness and psychological health: A pilot intervention.

OBJECTIVE: This study examined the differences in first-year college students' daily change trajectories in subjective happiness, depression, anxiety, stress, and state mindfulness. METHOD: A 5-day, online mindfulness-based intervention (MI) condition and stress management (SM) condition were employed in 247 first-year college students. RESULTS: Students in both the MI and SM conditions displayed similar significant linear declines in anxiety and stress, but there were significant differences between the two conditions, including: (1) the MI condition showing a significant linear increase in subjective happiness compared with no change in the SM condition and (2) the SM condition showed a significant linear decrease in depression compared to no significant change in the MI condition. CONCLUSION: Brief online interventions-whether MI or SM-can promote better mental health and reduce psychological distress. The results also lend support for MI's differential influence on first-year college students' happiness and SM's differential influence on their depression.

A review and meta-analysis of perfectionism interventions: Comparing face-to-face with online modalities.

Extending Lloyd, Schmidt, Khondoker, and Tchanturia (2015), this review and meta-analysis evaluated the effectiveness of randomized controlled trials aimed at reducing perfectionism and associated symptoms of depression and anxiety. Of particular interest was the examination of a moderator of delivery method (face-to-face vs. online) in testing the effectiveness of psychological interventions. Also examined is the effect of two structural moderators (control condition type, sample characteristic). A total of 10 studies were identified (65 perfectionism effect sizes, 8 depression effect sizes, and 8 anxiety effect sizes). Psychological interventions were effective in decreasing perfectionism dimensions, depression, and anxiety with medium effect sizes. There were no significant differences in delivery modality, control condition type, and sample characteristic on effect sizes. Findings suggest that psychological interventions are generally effective in reducing perfectionism, although there is no apparent delineation in increasing "adaptive" perfectionism and decreasing "maladaptive" perfectionism. Although comparable benefits were shown in face-to-face and online intervention delivery modes, we suggest prudently incorporating online interventions into clinical practice. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

The CES-D as a Measure of Psychological Distress Among International Students: Measurement and Structural Invariance Across Gender.

Detecting psychological distress among international students can be challenging given diverse languages, cultural backgrounds, and lack of refined measurement properties of measures tailored to international students. Despite the challenges, ensuring that a psychological distress measure works effectively has considerable potential value for assessment purposes. The current study evaluates the measurement properties of a short 10-item version of Radloff's Center for Epidemiologic Studies Depression Scale (CES-D). Grounded in long-standing evidence on gender differences in depressive symptoms, specific attention was given to examining measurement invariance of the CES-D Short-form across women and men. Based on a large, two-cohort sample of international students ( N = 468), and through multiple analyses evaluating factor structure and measurement invariance, we derived an even briefer, seven-item single-factor form of the CES-D (CES-D Short-form International) that can be used with international students.

The advising alliance for international and domestic graduate students: Measurement invariance and implications for academic stress.

We expanded the focus of a prior study of international graduate student advising relationships (Rice et al., 2009) to examine advising experiences of both international and domestic students. International (n = 434) and domestic (n = 387) students completed the Advisory Working Alliance Inventory (AWAI-S; Schlosser & Gelso, 2001) and measures of advising experiences, perceived academic stress, and desire to change advisor. Measurement invariance analyses suggested that a 23-item AWAI-S showed support for scalar invariance. A bifactor structure showed superior fit to the 3-factor model or a second-order factor model for the AWAI-S. International and domestic graduate students did not differ in ratings of general alliance, academic stress, or desire to change advisors. General alliance was strongly related to less academic stress and less desire to change advisors. International students who felt disrespected by their advisors were more likely to be academically stressed than domestic students. Structured mentoring experiences were associated with lower stress and less desire to change, and this effect was similar in both international and domestic students. Overall, results suggested that the current level of measurement, and possibly theory development, regarding the advisory alliance is good at identifying generic satisfaction but weaker at differentiating components of the alliance.

Factor structure of the perfectionistic self-presentation scale in Korea.

This study explored the factor structure of the Korean version of the Perfectionistic Self-Presentation Scale, originally developed by Hewitt and colleagues in 2003 with three factors (Perfectionistic Self-promotion, Non-display of Imperfection, and Non-disclosure of Imperfection). In Study 1, a confirmatory factor analysis was performed on the Korean version with 27 items for 151 Korean college students, but the model fit was poor. Subsequently, an exploratory factor analysis was conducted, and the results yielded three factors as found in Hewitt, et al., yet with 20 items rather than the original 27 items. This new version had good internal consistency (Cronbach's alpha =.88); convergent validity estimate was established with a measure of self-presentation motivation. In Study 2, to support the structural validity of the Korean version, another confirmatory factor analysis was conducted with 203 Korean college students. The model fit was good, but a few amendments were made.